Technical Data
W3006-02D
Wnt1 (Proto-oncogene Wnt-1, Proto-oncogene Int-1 Homolog, INT1)
Description:
Wnt1, a 370aa protein belonging to the Wnt family, is a signaling molecule important in CNS development. It plays a role in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt1 signaling is involved in many functions including the inhibition of apoptosis in several cancers, inhibition of interfollicular progenitor cell growth and it promotes terminal differentiation of keratinocytes and plays a role in angiogenesis by endothelial cell growth control that is mediated through cell-cell contact. Wnt1 blockade by either monoclonal antibody or siRNA induces cell death in sarcoma cells. It is expressed in hair follicles, sebaceous glands, skin and other tissues.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic peptide corresponding to aa275-288 (EPEDPAHKPPSPHD) of human Wnt1.
Purity:
Serum
Form
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Specificity:
Recognizes human Wnt1/Int1. Species Crossreactivity: bovine, canine, equine, monkey, opossum, rat. Species sequence homology: pufferfish, zebrafish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cheng, CW. et al. Exp. Cell. Res. 291:415-425 (2003).