Technical Data
W3006-02G
Wnt1 (Proto-oncogene Wnt-1, Proto-oncogene Int-1 Homolog, INT1)
Description:
Wnt gene family members, including Wnt-1 through Wnt-10, play a key role in regulating cellular growth and differentiation. Wnt-1 is a cysteine-rich, secreted glycoprotein that associates with cell membranes and likely functions as a key regulator of cellular adhesion. Wnt-1, which is essential for normal development of the embryonic nervous system, contributes to hyperplasia and tumorigenic progression when improperly expressed in mammary tissue. Wnt-3 is also involved in tumorigenesis and Wnt-2 and Wnt-4 may be associated with abnormal proliferation in human breast tissue. Wnt-1, Wnt-3 and Wnt-10b have been implicated along with FGF-3 in the development of mouse mammary tumor virus induced mouse mammary carcinomas.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-4ug/ml
Immunohistochemistry: 10-20ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa31 from human Wnt-1.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
Specificity:
Recognizes human Wnt-1 at ~41kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.