Technical Data
W3007
Wnt2B (Wingless-type MMTV (mouse mammary tumor virus) Integration Site Family, member 2B)
Description:
Wnts have diverse roles in governing cell fate, proliferation, migration, and polarity during development, particularly in embryogenesis and carcinogenesis in adults1-3. Wnts are transduced through at least three distinct intracellular signaling pathways, including the canonical Wnt/b-catenin, Wnt/Ca2+ and Wnt polarity (also called ‘planar polarity’)2, 4,5 pathways.
Distinct sets of Wnt and Frizzled ligand-receptor pairs activate each of these pathways and lead to unique cellular responses. The Wnt/b-catenin pathway primarily regulates cell fate determination during development, whereas the main function of the Wnt polarity pathway is the regulation of cytoskeletal organization. Wnt2B/Wnt13 is expressed in the embryonic mesoderm during gastrulation, and in the dorsal midline of the diencephalons and mesencephalon, the heart primordial, the lung bud periphery, and in the otic and optic vesicles at later stages.6 Wnt2B is expressed in organs regulated by epithelial-mesenchymal interactions in the mouse embryo, such as the developing kidney, lung, salivary gland, gut, pancreas, adrenal gland, genital tubercle, eye and ear.7 Wnt2B signaling induces ureter branching during early kidney organogenesis.7 Wnt2B exists as 2 alternatively spliced isoforms (Wnt2B1 and Wnt2B2).8 Wnt2B2 is preferentially expressed in NT2 cells, with the potential of neuronal differentiation.9 In some cases of gastric cancer, Wnt2B2 up-regulation may lead to carcinogenesis through the activation of the b-catenin/TCF signaling pathway.9 Wnt2B is expressed in the immature ovary, where the Wnt signaling cascade may be involved in follicular development and ovarian function.10

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blotting: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
0.25mg/ml
Immunogen:
Synthetic peptide derived from an internal region of the human Wnt2B/Wnt13 protein.
Purity:
Epitope-affinity purified from rabbit antiserum.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Reacts with the human, mouse and rat Wnt2B/Wnt13 proteins. It recognizes both human Wnt13A and Wnt13B isoforms. On Western blots, it identifies a band at ~41kD. Reactivity has been confirmed with mouse Wnt2B/Wnt13-pCMV-tag2-transfected 293T, human Wnt13A- and Wnt13B-pCR3-transfected HEK293 and SK-OV-3 cell lysates, and rat ovary homogenates.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cadigan KM and Nusse R. Genes Dev 11:3286-3305, 1997.
2. Miller JR, et al. Oncogene 18:7860-7872, 1999.
3. Polakis P. Genes Dev 14:1837-1851, 2000.
4. Adler, PN and Lee H. Curr Opin Cell Biol 13:635-640, 2001.
5. Kuhl M, et al. Trends Genet 16:279-283, 2000.
6. Zakin LD, et al. Mech Dev 73(1):107-116, 1998.
7. Lin Y, et al. Dev Dyn 222(1):26-39, 2001.
8. Katoh M, et al. Biochem Biophys Res Commun 275(1):209-216, 2000.
9. Katoh M, et al. Biochem Biophys Res Commun 289(5):1093-1098, 2001.
10. Ricken A, et al. Endocrinology 143(7):2741-2749, 2002.