Technical Data
W3021-01C
Wnt4, ID (Protein Wnt-4, UNQ426/PRO864)
Description:
Wnt-4 belongs to the WNT family of signaling molecules that have vital roles in the development of several tissues within invertebrates and vertebrates, serving as binding ligands for frizzled family transmembrane receptors. A secreted glycoprotein, Wnt-4 is a necessary component of nephrogenesis and metacephric mesenchymal condensation. It also has been shown to be involved in unilateral uretal obstruction (UUO). Some studies suggest Wnt-4 to have a potential usefulness in the improvement of bone regeneration and restoration of craniofacial defects. Expression of Wnt-4 is wide and has been observed in kidney, adrenal gland, gonad, mammary gland, Mullerian duct, and pituitary gland. A deficiency of Wnt-4 has been implicated in causing partial female to male sex reversal, known as SERKAL syndrome. Defects in Wnt-4 expression are also causal to Rokitansky-Kuster-Hauser syndrome (RKH syndrome), which is characterized by anomalies in the genital tract of otherwise normal females.

Applications:
Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.

Recommended Dilutions:
Western Blot: 2ug/ml detected WNT4 on 10ug of human testis tissue lystae
Immunohistochemistry (Paraffin): 1:300 detected WNT4 in human testis tissue lystae
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Linear peptide corresponding to the mature chain of human protein Wnt-4, conjugated to KLH. (Accession #P56705), Species Sequence Homology: canine.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human protein Wnt-4 at ~35kD. Species Crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.