		Technical Data
W3035-55B	WNT3 (Proto-oncogene Wnt-3, Proto-oncogene Int-4 Homolog, INT4)

Description:
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 1-3ug/ml, observed in human brain lysates on ~38kD bands
Immunohistochemistry (Paraffin): 3-5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to CGRGHNTRTEKRKEK, from WNT3, at the internal region of the protein (NP_110380.1).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Specificity:

Recognizes human WNT3. Species Crossreactivity: Mouse. Species sequence homology: Canine and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Okamoto M, Inoue K, Iwamura H, Terashima K, Soya H, Asashima M, Kuwabara T. Reduction in paracrine Wnt3 factors during aging causes impaired adult neurogenesis. FASEB J. 2011 Jul 11.