Technical Data
W3035-92C
Wnt5B (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member 5B, Wnt 5B, Wnt-5B)
Description:
WNT5B is a ligand for members of the frizzled family of seven transmembrane receptors. Wnt proteins constitute a large family of secreted molecules that are involved in intercellular signalling during development and have been implicated in oncogenesis. Very little is known about the structure of Wnts as they are notoriously insoluble; but they share the following features characteristics of secretory proteins: a signal peptide, several potential N-glycosylation sites and 22 conserved cysteines. The Wnt proteins seem to adhere to the plasma membrane of the secreting cells and are therefore likely to signal over only few cell diameters. Fifteen major Wnt gene families have been identified in vertebrates, with multiple subtypes within some classes.

Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:200,000-1:300,000
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Length (aa): 359
Mol. Weight (Da): 40323

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide, KLH conjugated human WNT5B. Epitope: Internal domain
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 2% sucrose. Reconstitute with 100ul ddH2O.
Specificity:
Recognizes human WNT5B protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.