Technical Data
W3035-93
Wnt5b, ID (Protein Wnt-5b, wnt5b, wnt-5, wnt5, wnt5a, si:ch73-211c7.1)
Description:
WNT5B is a ligand for members of the frizzled family of seven transmembrane receptors. Wnt proteins constitute a large family of secreted molecules that are involved in intercellular signalling during development and have been implicated in oncogenesis. Very little is known about the structure of Wnts as they are notoriously insoluble; but they share the following features characteristics of secretory proteins: a signal peptide, several potential N-glycosylation sites and 22 conserved cysteines. The Wnt proteins seem to adhere to the plasma membrane of the secreting cells and are therefore likely to signal over only few cell diameters. Fifteen major Wnt gene families have been identified in vertebrates, with multiple subtypes within some classes.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
ELISA: 1:5000-1:20,000
Optimal dilutions to be determined by the researcher.

Control Peptide: W3035-93P

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceZebrafishRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to the intermediate region of zebrafish Wnt-5b.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.
Specificity:
Recognizes zebrafish Wnt-5b.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.