Technical Data
W3038-10B
Wnt10B (Protein Wnt-10b, Protein Wnt-12, Wnt12)
Description:
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT10B is a member of the WNT family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa81-93 (HECQHQLRDQRWN) of human Wmt10B.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes human Wnt10B. Species Crossreactivity: bovine, chimpanzee, canine, mouse, orangutan, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Expression of all Wnt genes and their secreted antagonists during mouse blastocyst and postimplantation development.Kem p C, Willems E, Abdo S, Lambiv L, Leyns L. Dev Dyn. 2005 Jul;233(3):1064-75. 2. Expression and regulation of WNT10B in human cancer: up-regulation of WNT10B in MCF-7 cells by beta-estradiol and down-regulation of WNT10B in NT2 cells by retinoic acid. Kirikoshi H, Katoh M. Int J Mol Med. 2002 Oct;10(4):507-11.