Technical Data
X-Gal (5-Bromo-4-chloro-3-indolyl-b-D-galactoside galactopyranoside, Xgal)
Biochemicals Storage: -20°CShipping: Blue Ice
Blue substrate used in the detection of b-galactosidase in bacteria or phage as a selection agent for cloning experiments utilizing the lacZ vector. Colonies expressing b-galactosidase will appear blue in the presence of XGAL. Others will appear white.

Purity (HPLC): 99%
Purity (TLC): Single Spot
Appearance: White lyophilized powder
Solubility: Soluble in DMF
Water (KF): 1%
Specific Rotation: -60º to -64º
Identity by I.R. or NMR: Conforms to structure
RNase, DNase or Proteases: None Detected

Quality Control (Blue-White Assay):
[LacZ+]: Pale blue center/dense blue periphery
[LacZ-]: Faint blue center/white periphery

Storage and Stability:
Store at -20°C. Stable for 6 months. Protect from light.

Also Available:
X1000: XGAL
X1000-05: XGAL, 40mg/ml in DMF

CAS Number:

Molecular Formula:

Molecular Weight:
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
US Biological application references: 1. Hwang, L.H and Murray, A.W. (1997) Mol. Biol. Cell 8:1877-1887. 2. Tsuchida, T. et al., (2004) Biotechniques 37:896-897. 3. Coombs, J.M and Brenchley, J.E. (1999) Applied and Environmental Microbiology, 65:5443-5450. 4. Itkin-Ansari, P. et al., (2005) Developmental Dynamics 233:946–953. 5. Hamouda, T. et al., (1997) J. Virol., 71:5521-5527. 6. Hisa, T. et al., (2004) EMBO J. 23:450-459. 7. Gärtner, U. et al., (20040 J Neurosci Res. 77:630-641. 1. Horvitz, J.P., et al., J. Med. Chem. 7: 574 (1964). 2. Lin, W.C., et al., Cancer Res. 50: 2808-2817 (1990). 3. Buckner, F.S., et al., Infect. Immun. 67: 403-409 (1999). 4. Weiss, D.J., et al., Hum. Gene Ther. 8: 1545-1554 (1997). 5. Sambrook, J., et al., Molecular Cloning: A Laboratory Manual, Plainview, NY, 1.85-1.86 and B.14 (1989).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.