X-Gal (5-Bromo-4-chloro-3-indolyl-b-D-galactoside galactopyranoside, Xgal)
|Biochemicals||Storage: -20°CShipping: Blue Ice|
Blue substrate used in the detection of b-galactosidase in bacteria or phage as a selection agent for cloning experiments utilizing the lacZ vector. Colonies expressing b-galactosidase will appear blue in the presence of XGAL. Others will appear white.
Purity (HPLC): 99%
Purity (TLC): Single Spot
Appearance: White lyophilized powder
Solubility: Soluble in DMF
Water (KF): 1%
Specific Rotation: -60º to -64º
Identity by I.R. or NMR: Conforms to structure
RNase, DNase or Proteases: None Detected
Quality Control (Blue-White Assay):
[LacZ+]: Pale blue center/dense blue periphery
[LacZ-]: Faint blue center/white periphery
Storage and Stability:
Store at -20°C. Stable for 6 months. Protect from light.
X1000-05: XGAL, 40mg/ml in DMF
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.