Technical Data
X1000
X-Gal (5-Bromo-4-chloro-3-indolyl-b-D-galactoside (galactopyranoside)
100mg
250mg
1g
10g
250mg
1g
10g
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Technical Data |
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X1000 |
X-Gal (5-Bromo-4-chloro-3-indolyl-b-D-galactoside (galactopyranoside) |
100mg 250mg 1g 10g |
| Biochemicals | Storage: -20°CShipping: Blue Ice |
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Blue substrate used in the detection of b-galactosidase in bacteria or phage as a selection agent for cloning experiments utilizing the lacZ vector. Colonies expressing b-galactosidase will appear blue in the presence of XGAL. Others will appear white. Purity (HPLC): 99% Purity (TLC): Single Spot Appearance: White lyophilized powder Solubility: Soluble in DMF Water (KF): 1% Specific Rotation: -60º to -64º Identity by I.R. or NMR: Conforms to structure RNase, DNase or Proteases: None Detected Quality Control (Blue-White Assay): [LacZ+]: Pale blue center/dense blue periphery [LacZ-]: Faint blue center/white periphery Storage and Stability: Store at -20°C. Stable for 6 months. Protect from light. Also Available: X1000: XGAL X1000-05: XGAL, 40mg/ml in DMF CAS Number: 7240-90-6 Molecular Formula: C14H15BrClNO6 Molecular Weight: 408.61 |
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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US Biological application references: 1. Hwang, L.H and Murray, A.W. (1997) Mol. Biol. Cell 8:1877-1887. 2. Tsuchida, T. et al., (2004) Biotechniques 37:896-897. 3. Coombs, J.M and Brenchley, J.E. (1999) Applied and Environmental Microbiology, 65:5443-5450. 4. Itkin-Ansari, P. et al., (2005) Developmental Dynamics 233:946–953. 5. Hamouda, T. et al., (1997) J. Virol., 71:5521-5527. 6. Hisa, T. et al., (2004) EMBO J. 23:450-459. 7. Gärtner, U. et al., (20040 J Neurosci Res. 77:630-641. 1. Horvitz, J.P., et al., J. Med. Chem. 7: 574 (1964). 2. Lin, W.C., et al., Cancer Res. 50: 2808-2817 (1990). 3. Buckner, F.S., et al., Infect. Immun. 67: 403-409 (1999). 4. Weiss, D.J., et al., Hum. Gene Ther. 8: 1545-1554 (1997). 5. Sambrook, J., et al., Molecular Cloning: A Laboratory Manual, Plainview, NY, 1.85-1.86 and B.14 (1989).
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