Technical Data
X1000-05
X-Gal, 40mg/ml in DMF (5-Bromo-4-chloro-3-indolyl-b-D-galactoside galactopyranoside, Xgal)
10ml
5x10ml
Biochemicals Storage: -20CShipping: Blue Ice
Blue substrate used in the detection of b-galactosidase in bacteria or phage as a selection agent for cloning experiments utilizing the lacZ vector. Colonies expressing b-galactosidase will appear blue in the presence of XGAL. Others will appear white.

Purity (HPLC): 99%
Purity (TLC): Single Spot
Concentration: ~40mg/ml
Form: Supplied as a liquid in dimethylformamide.
Solubility: Colorless to pale yellow, clear, complete
Identity by I.R. or NMR: Conforms to structure

RNase, DNase or Proteases: None Detected

Quality Control (Blue-White Assay):
[LacZ+]: Pale blue center/dense blue periphery
[LacZ-]: Faint blue center/white periphery

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Storage and Stability:
Store at -20C. Aliquots are stable for 12 months. Protect from light.

Also Available:
X1000: XGAL
X1000-05: XGAL, 40mg/ml in DMF

CAS Number:
7240-90-6

Molecular Formula:
C14H15BrClNO6, DMF

Molecular Weight:
408.61
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Horvitz, J.P., et al., J. Med. Chem. 7:574 (1964). 2. Lin, W.C., et al., Bacterial lacZ gene as a highly sensitive marker to detect micrometastasis formation during tumor progression. Cancer Res. 50:2808-2817 (1990). 3. Buckner, F.S., et al., Detection of live Trypanosoma cruzi in tissues of infected mice by using histochemical stain for beta-galactosidase. Infect. Immun. 67:403-409 (1999) 4. Weiss, D.J., et al., In situ histochemical detection of beta-galactosidase activity in lung. Hum. Gene Ther. 8:1545-1554 (1997). 5. Sambrook, J., et al., Molecular Cloning: A Laboratory Manual , Plainview, NY (1989), 1.85-1.86 and B.14.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.