Technical Data
X1027
XGLUC, Sodium Salt
100mg
500mg
1g
Biochemicals Storage: 4CShipping: RT
Indigo-blue chromogenic substrate used in the detection of -glucuronidase in bacterial colonies.

The chromogenic substrate X-Gluc is used in a variety of applications for the detection of the $-glucuronidase enzyme. Upon reduction, X-Gluc produces a localized color, making it useful in identifying GUS gene presence in most cell types1 and for the detection of the GUS gene fusion marker in plants.2,3 X-Gluc has reported applications in the detection of contaminated food
samples such as meat, dairy, and shellfish products.4,5 X-Gluc also has clinical applications in the assessment of urinary tract infection by d etecting the pr esence of E. coli.6 It has gained international acceptance as an accurate indicator for the presence of E. coli in potable water samples b y reducing false positives and negatives found with traditional methods.

BENEFITS
-Saves time compared to other E. coli detection methods... 24 hour direct plating method7,9,11
-Minimizes errors...less than 1% false negatives and less than 5% false positives7,8,9
-Easy visual d etection...8max is in visible range
-Sensitive for p resence/ab sence of E. coli...eliminates limitation s of very rapid presence/absence or chemiluminometric methods12

Applications:
Suitable for use in environmental testing of E. coli in potable water, detection of GUS expression in the study of trans cription, translation, and protein transport events, determination of urinary tract infections and identifying food products contaminated with E. coli

Specific Rotation (C=1, H2O:DMF 1:1): -107 to -110
Solubility (2% DMF): Colorless, clear, complete
Water (KF): ~10%
H-NMR: Conform to structure
DNase or Proteases:
None Detected

CAS Number:
129541-41-9

Molecular Formula:
C14H12BrClNNaO7

Molecular Weight:
444.7
Purity: 98%
Form: White crystalline powder.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
US Biological application reference: Behari, J. and Youngman, P. (1998) J. Bacteriology 180:6316-6324. 1. Ellis, D.D., et al., Bio/Technology, 1993, 11 (1), 84-89 2. Bommineni, V.R., et al., Plant Cell Reports, 1993, 13 (1), 17-23 3. Martin, G.C., et al., J. Amer. Soc. Horticult. Sci., 1990, 115 (46), 686-691 4. Restaino, L., et al., J. Food Protect., 1990, 53 (6), 508-510 5. Watkins, W.D., et al., Appl. Environ. Microbiol., 1988, 54, 1874-1875 6. Delisle, G.J., and Ley, A., J. Clin. Microbiol. 1989, 27 (4), 778-779 7. Gaudet, I.D., et al., Appl. Environ. Microbiol., 1996, 62 (11), 4032-4035 8. Ceibin, B.W., et al., Appl. Environ. Microbiol., 1995, 61 (11), 3940-3942 9. Sartory, D.P., and Howard, L., Letters in Applied Microbiology, 1992, 15, 2373-2376 10. Brenner, K.P., et al., Abstracts for the Annual Meeting of the American Society of Microbiology, 1993 11. Frampton, E.W., et al., J. Food Protect. 1988, 51 (5), 402-404 12. Van Pouckle, S.O., and Nelis, H.J., Appl. Environ. Microbiol., 1997, 63 (2), 771-774

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.