Technical Data
X9501-06
XRCC1 (X-Ray Repair Cross Complementing Protein1)
Description:
X-Ray Repair Cross Complementing (XRCC1) plays a role in excision repair of DNA after ionizing irradiation. XRCC1 binds to DNA ligase III through the C-terminal 96 amino acids and to DNA polymerase beta through the N-terminal half.. In testis XRCC1 is expressed at high levels. Cells with a mutation of this gene exhibit decreased single strand break repair and reduced recombination repair. They show increased double strand breaks, and sister chromatid exchange is increased up to 10-fold. The XRCC1 might serve as a scaffold protein during base excision-repair.

Applications:
Immunohistochemistry: 1:100-1:500. Frozen
Immunoprecipitation
Western Blotting: 1:1000-1:10,000. Detects a band of approximately 90kD

Recommended Secondary Reagents
Sheep anti Rabbit IgG (Aff. Pur.): HRP

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
1mg/ml
Immunogen:
Peptide corresponding to a region of human XRCC1 encoded within exon 17
Purity:
Purified by affinity chromatography
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes human XRCC1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.