Technical Data
Media
Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate (YNB) (Powder)
Y2020
Molecular Biology Grade
Storage RT/4°C    Shipping RT
Components per liter:
Nitrogen Source:
Ammonium Sulfate5g
Amino Acids:
L-Histidine•HCl10mg
DL-Methionine20mg
DL-Tryptophan20mg
Vitamins:
p-Aminobenzoic acid200ug
Biotin2ug
Folic Acid2ug
Inositol2000ug
Niacin400ug
D-Pantothenic Acid•Ca400ug
Pyridoxine•HCl400ug
Riboflavin200ug
Thiamine•HCl4400ug
Trace Elements:
Boric Acid500ug
Copper Sulfate40ug
Ferric Chloride200ug
Manganese Sulfate400ug
Potassium Iodide100ug
Sodium Molybdate200ug
Zinc Sulfate400ug
Inorganic Salts:
Calcium Chloride0.1g
Magnesium Sulfate0.5g
Potassium Phosphate Monobasic1g
Sodium Chloride0.1g
Total:6.756g/L
500g makes 74 liters
Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). Their research on the nutritional requirements of yeast strains resulted in the formulation of YNB. YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae.


Directions per Liter: Dissolve 6.76 g in 800-900ml of ddH2O, stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving. Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Precautions:Yeast Nitrogen Base contains heat-labile compounds that can be damaged with autoclaving. This product should be sterilized with a 0.2µ filter.

Appearance: White to off-white, homogeneous, free-flowing powder

Solubility: Colorless to pale yellow, clear, complete

pH: 5.0 ± 0.5

Precautions: Yeast Nitrogen Base contains heat-labile compounds that can be damaged with autoclaving. This product should be sterilized with a 0.2µ filter. Important Note


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Difco Manual Tenth Edition, pp.1136-1141(1984). 2. Ronald M. Atlas, Handbook of Microbiological Media, Lawrence C. Parks, ed., CRC Press (1993).