Technical Data
Media
Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate w/o Fe, Cu (YNB) (Powder)
Y2021
Molecular Biology Grade
Storage RT/4°C    Shipping RT
Components per liter:
L-Histidine•HCl10mg
DL-Methionine20mg
DL-Tryptophan20mg
p-Aminobenzoic acid200ug
Biotin2ug
Boric Acid500ug
Copper SulfateAbsent
Ferric ChlorideAbsent
Folic Acid2ug
Inositol2000ug
Manganese Sulfate400ug
Niacin400ug
D-Pantothenic Acid•Ca400ug
Potassium Iodide100ug
Pyridoxine•HCl400ug
Riboflavin200ug
Sodium Molybdate200ug
Thiamine•HCl400ug
Zinc Sulfate400ug
Ammonium Sulfate5g
Calcium Chloride0.1g
Magnesium Sulfate0.5g
Potassium Phosphate Monobasic1g
Sodium Chloride0.1g
Total:6.76g/L
500g makes 74.5 liters
Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). Their research on the nutritional requirements of yeast strains resulted in the formulation of YNB. YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae.

Application: For carbon assimilation tests in the characterization and identification of yeasts.

Note: The addition of a carbon source like glucose, galactose or raffinose is required.


Directions per Liter: Dissolve 6.76g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Note: The addition of a carbon source like glucose, galactose or raffinose is required.

Appearance: White to pale yellow, homogeneous, free flowing powder

Solubility: Colorless to pale yellow, clear, complete

pH: As Reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Difco Manual Tenth Edition, pp.1136-1141(1984). 2. Ronald M. Atlas, Handbook of Microbiological Media, Lawrence C. Parks, ed., CRC Press (1993).