Technical Data
Media
Yeast Nitrogen Base w/o AA, Carbohydrate & w/o AS (YNB) (Powder)
Y2030
Molecular Biology Grade
Storage RT/4°C    Shipping RT
Components shown as mg/liter:
Potassium Phosphate
Monobasic Anhydrous1000
Magnesium Sulfate500
Sodium Chloride100
Calcium Chloride100
Components shown as ug/liter:
Biotin2
D-Pantothenic Acid•Ca400
Folic Acid2
Inositol2000
Niacin400
p-Aminobenzoic acid200
Pyridoxine•HCl400
Riboflavin200
Thiamine•HCl400
Boric Acid500
Copper Sulfate40
Potassium Iodide100
Ferric Chloride200
Manganese Sulfate400
Sodium Molybdate200
Zinc Sulfate400
Total:1.72g/L
500g makes 294 liters
Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae.
Y2030 has the same formulation as YNB without Amino Acids except that ammonium sulfate as a source of nitrogen has been omitted to test the ability of yeast cells to assimilate different nitrogen sources (i.e. Potassium Nitrate).

Application: For carbon assimilation tests in the characterization and identification of yeasts.

Note: The addition of a carbon source like glucose, galactose or raffinose is required.


Directions per Liter: Dissolve 1.72g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Off-white to light yellow, homogeneous free-flowing powder.

Solubility: Colorless to pale yellow, clear, complete

pH: 4.7±0.6

Note: The addition of a carbon source like glucose, galactose or raffinose is required.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

US Biological application reference: Gander, S. et al., (2008) Rapid Commun Mass Spectrom. 22:3743-3753. 1. Difco Manual Tenth Edition, pp.1136-1141 (1984). 2. Ronald, M., Atlas, Handbook of Microbiological Media, Lawrence C. Parks, ed., CRC Press (1993).