Technical Data
Media
Yeast Nitrogen Base w/AA & w/AS, w/o Vitamins (YNB) (Powder)
Y2035
Molecular Biology Grade
Storage RT/4°C    Shipping RT
Components mg/Liter:
Nitrogen Source:
Ammonium Sulfate5000
Dextrose10000
Amino acids:
L-Histidine•HCl10
DL-Methionine20
DL-Tryptophan20
Vitamins:
BiotinAbsent
D-Pantothenic Acid•CaAbsent
Folic AcidAbsent
InositolAbsent
NiacinAbsent
p-Aminobenzoic acidAbsent
Pyridoxine•HClAbsent
RiboflavinAbsent
Trace Elements:
Boric Acid0.5
Copper Sulfate0.04
Ferric Chloride0.2
Manganese Sulfate0.4
Potassium Iodide0.1
Sodium Molybdate0.2
Zinc Sulfate0.4
Inorganic Salts:
Calcium Chloride100
Magnesium Sulfate500
Potassium Phosphate Mono1000
Sodium Chloride100
Total16.7g/L
Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae. Y2035 is based upon the formulation of YNB except that all vitamins are omitted. The medium is used to test the requirement of essential vitamins.

Application: For carbon assimilation tests in the characterization and identification of yeasts.


Directions per Liter: Dissolve 16.7 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White to tan, homogeneous, free-flowing powder

Solubility: Colorless, clear, complete

pH: 4.5 ± 0.5


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

US Biological application reference: Edwards, C.G and J.C. Bohlscheid, J.C. (2007) Enzyme and Microbial Technology 41:1-4. 1. Difco Manual Tenth Edition, pp.1136-1141(1984). 2. Ronald M. Atlas, Handbook of Microbiological Media, Lawrence C. Parks, ed., CRC Press (1993).