Technical Data
Media
Yeast Nitrogen Base w/o AA & w/o AS, w/o Vitamins (YNB) (Powder)
Y2035-01
Molecular Biology Grade
Storage RT/4C    Shipping RT
Yeast Nitrogen Base w/o AS, Vitamins, AA
Components per liter:
Trace Elements:
Boric Acid500ug
Copper Sulfate40ug
Ferric Chloride200ug
Manganese Sulfate400ug
Potassium Iodide100ug
Sodium Molybdate200ug
Zinc Sulfate400ug
Inorganic Salts:
Calcium Chloride0.1g
Magnesium Sulfate0.5g
Potassium Phosphate Monobasic1g
Sodium Chloride0.1g
Total1.701 g/L
Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). Their research on the nutritional requirements of yeast strains resulted in the formulation of YNB. YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae.

Y2035-01 is based upon the formulation of YNB except that all vitamins are omitted. The medium is used to test the requirement of essential vitamins.

Application: For carbon assimilation tests in the characterization and identification of yeasts.


Directions per Liter: Dissolve 1.70 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: White to tan, homogeneous, free-flowing powder

Solubility: Colorless to yellow, clear, complete

pH: As Reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Difco Manual Tenth Edition, pp.1136-1141(1984). 2. Ronald M. Atlas, Handbook of Microbiological Media, Lawrence C. Parks, ed., CRC Press (1993).