Technical Data
Media
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Technical Data
Media
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Yeast Nitrogen Base w/o AA & w/o AS, w/o Vitamins (YNB) (Powder)
Y2035-01
Molecular Biology Grade
Storage RT/4°C Shipping RT
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Yeast Nitrogen Base w/o AS, Vitamins, AA
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Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). Their research on the nutritional requirements of yeast strains resulted in the formulation of YNB. YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae. Y2035-01 is based upon the formulation of YNB except that all vitamins are omitted. The medium is used to test the requirement of essential vitamins. Application: For carbon assimilation tests in the characterization and identification of yeasts. Directions per Liter: Dissolve 1.7g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving. Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time. Appearance: White to tan, homogeneous, free-flowing powder Solubility: Colorless to yellow, clear, complete pH: As Reported Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Difco Manual Tenth Edition, pp.1136-1141(1984). 2. Ronald M. Atlas, Handbook of Microbiological Media, Lawrence C. Parks, ed., CRC Press (1993). |