Technical Data
Media
Yeast Nitrogen Base (YNB) w/AA & w/AS, Vitamin Free, w/o Dextrose (Powder)
Y2035-05
Molecular Biology Grade
Storage RT/4°C    Shipping RT
Components mg/Liter:
Nitrogen Source:
Ammonium Sulfate5000
DextroseAbsent
Amino acids:
L-Histidine•HCl10
DL-Methionine20
DL-Tryptophan20
Vitamins:
BiotinAbsent
D-Pantothenic Acid•CaAbsent
Folic AcidAbsent
InositolAbsent
NiacinAbsent
p-Aminobenzoic acidAbsent
Pyridoxine•HClAbsent
RiboflavinAbsent
Thiamine•HClAbsent
Trace Elements:
Boric Acid0.5
Copper Sulfate0.04
Ferric Chloride0.2
Manganese Sulfate0.4
Potassium Iodide0.1
Sodium Molybdate0.2
Zinc Sulfate0.4
Inorganic Salts:
Calcium Chloride100
Magnesium Sulfate500
Potassium Phosphate Mono1000
Sodium Chloride100
Total6.7g/L
100g makes 15 liters
Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). Their research on the nutritional requirements of yeast strains resulted in the formulation of YNB. YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae.
Y2035-05 is based upon the formulation of YNB except that all vitamins are omitted. The medium is used to test the requirement of essential vitamins.


Directions per Liter: Dissolve 6.7 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White to off-white, homogeneous, free-flowing powder Solubility


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Difco Manual Tenth Edition, pp.1136-1141(1984). 2. Ronald M. Atlas, Handbook of Microbiological Media, Lawrence C. Parks, ed., CRC Press (1993)