Technical Data
Yeast Nitrogen Base w/o AA & w/AS (YNB) Low Fe, Zn, Mn, Cu (Powder)
Molecular Biology Grade
Storage RT/4°C    Shipping RT
Components per Liter:
p-Aminobenzoic Acid200ug
Ammonium Sulfate5g
Boric Acid500ug
Calcium Chloride0.1g
Folic Acid2ug
Magnesium Sulfate0.5g
Pyridoxine HCl400ug
D-Pantothenic Acid•Ca400ug
Potassium Iodide100ug
Potassium Phosphate Mono1g
Sodium Chloride0.1g
Sodium Molybdate200ug
Thiamine HCl400ug
Total: 6.705g/L
500g makes 74.5 liters
Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). Their research on the nutritional requirements of yeast strains resulted in the formulation of YNB. YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae.

Application: For carbon assimilation tests in the characterization and identification of yeasts.

Note: The addition of a carbon source like glucose, galactose or raffinose is required.

Directions per Liter: Dissolve 6.7g in 800-900ml ddH2O of ddH2O. Heat with stirring until completely solubilized. Adjust pH as necessary. Add additional water to bring the solution to1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Note: The addition of a carbon source like glucose, galactose or raffinose is required.

Appearance: White to off-white, homogeneous, free-flowing powder

Solubility: Colorless to pale yellow, clear, complete

pH: As Reported

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Roberts, L. A. et al., (2004) Plant Physiology 135:112-120. 1. Difco Manual Tenth Edition, pp.1136-1141(1984). 2. Ronald M. Atlas, Handbook of Microbiological Media, Lawrence C. Parks, ed., CRC Press (1993).