Technical Data
Media
YEP Broth (Powder)
Y2050
Molecular Biology Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Tryptone20g
Yeast Extract10g
500g makes 16.7 liters
Standard media base for the maintenance and propagation of Schizosaccharomyces pombe and Saccharomyces cerevisiae. YEP Broth is YPD (YEPD) without dextrose and is used as a base for making a variation of YPD with an alternate carbon source.


Directions per Liter: Dissolve 30 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: Tan to light yellow homogeneous, free flowing powder

Solubility: Yellow to amber, clear, complete

pH: As Reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.