Technical Data
YPD Agar Traditional Formulation w/Peptone (Powder)
Molecular Biology Grade
Storage RT    Shipping RT
Components shown as g/liter:
Yeast Extract10
500g makes 7.5 liters
Standard formulation of YPD agar, using peptone. For the maintenance and propagation of yeasts. Also useful in plating of mutants and transformants.

Directions per Liter: Dissolve 67 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Light tan, homogeneous, free flowing powder Solubility

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Toulmay, A. and Schneiter, R. (2006) Yeast 23:825-831.