Technical Data
Media
YPD Agar Enhanced Formulation w/Peptone Y, Adenine (Powder)
Y2072
Molecular Biology Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Yeast Extract10g
Peptone Y20g
Dextrose20g
Agar17g
Adenine Hemisulfate120mg
Total:67.12g/L
500g makes 7.5 liters
Alternate formulation of the YPD series media, formulated with Peptone Y (casein digest, tryptone), useful for yeast propagation and plating of mutants and transformants.

Also includes the addition of an adenine hemisulfate supplement to a final 1X concentration of 120mg/L. The adenine supplement prevents yeast strains harboring ADE1 and ADE2 mutations (e.g. Y2HGold, AH109, and Y187) from turning pink/red after extended growth.


Directions per Liter: Dissolve 67.12g per liter of ddH2O. Heat with stirring until completely solubilized. Adjust pH as necessary. Dispense into appropriate containers. Loosen caps or cover with foil. Autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: White to light tan, homogeneous, free flowing powder

Solubility: Amber, clear, complete after autovclaving

pH: As reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.