Technical Data
Media
YPD Broth Enhanced Formulation w/Peptone Y (Powder)
Y2075
Molecular Biology Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Yeast Extract10
Peptone Y20
Dextrose20
Total:50g/L
500g makes 10 liters
Alternate formulation of the YPD series media, formulated with Peptone Y (casein digest, tryptone), useful for yeast propagation and plating of mutants and transformants.


Directions per Liter: Dissolve 50g in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: Light yellow to tan, homogeneous, free flowing powder

Solubility: Yellow-amber, clear, complete

pH: 6.0-7.2


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

US Biological application references: 1 Koffel, R. et al., (2005) Molecular and Cellular Biology, 25:1655-1668. 2. Jacquier, N. and Schneiter, R. (2010) J. Cell Science 123; 2218-2227.