Technical Data
Media
YPD Broth Enhanced Formulation w/Peptone Y, Adenine (Powder)
Y2077
Molecular Biology Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Adenine Hemisulfate120mg
Dextrose20
Peptone Y20
Yeast Extract10
Total:50g/L
500g makes 10 liters
Alternate formulation of the YPD series media, formulated with Peptone Y (casein digest, tryptone), useful for yeast propagation and plating of mutants and transformants.

Also includes the addition of an adenine hemisulfate supplement to a final 1X concentration of 120mg/L. The adenine supplement prevents yeast strains harboring ADE1 and ADE2 mutations (e.g. Y2HGold, AH109, and Y187) from turning pink/red after extended growth.


Directions per Liter: Dissolve 50 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Light yellow to tan, homogeneous, free flowing powder

Solubility: Yellow-amber, clear, complete

pH: 6.0-7.2


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.