Technical Data
Z0328-50
ZBP1 (Z-DNA Binding Protein 1, C20orf183, DAI, dJ718J7.3, DLM1, DLM-1, Tumor Stroma and Activated Macrophage Protein DLM-1, DNA-dependent activator of IRFs)
Description:
Initial studies suggested that DAI may be involved in processes such host response to neoplasia, additional research has indicated that the cytosolic DAI can act like the toll-like receptor TLR9 by detecting cytosolic double-stranded (ds) DNA and trigger induction of type I interferon and other innate immune responses. It is thought that the binding of DAI to dsDNA enhances its association with innate immune response proteins such as the IRF3 transcription factor and the serine/threonine kinase TBK1 (also known as NAK).

DAI is highly expressed in lymphatic tissues such as the lymph node, leukocytes, tonsil, bone marrow and spleen. Expression at a lower level is also seem in thymus, lung and liver. It has been demonstrated that multiple isoforms of DAI exist.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to an 18aa sequence from near the carboxy terminus of human.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human DAI. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Takaoda A et al (2007) DAI (DLM-1/ZBP1) is a cytosolic DNA sensor and an activator of innate immune response. Nature 448:501-5. 2. Schwartz T, et al. (2001) Structure of the DLM-1-Z-DNA complex reveals a conserved family of Z-DNA-binding proteins. Nat. Struct. Biol. 8:761-5.