Technical Data
Z0400-03
ZBP1 (Z-DNA binding protein 1, C20orf183, DAI, dJ718J7.3, DLM1, DLM-1, Tumor stroma and activated macrophage protein DLM-1)
Description:
yZ-DNA binding protein 1 (ZBP1) belongs to a family of proteins that contain the Z domain which binds specifically to left-handed Z-DNA and Z-RNA (1). ZBP1 was initially identified as a novel gene that was up-regulated in activated macrophages in mice bearing ascites tumors, suggesting that is may play a role in processes such as host response in neoplasia (2). More recent reports indicate that the cytosolic ZBP1 has can act like the toll-like receptor TLR9 by detecting cytosolic double-stranded (ds) DNA and trigger induction of type I interferon and other innate immune responses. It is thought that the binding of ZBP1 to dsDNA enhances its association with innate immune response proteins such as the IRF3 transcription factor and the serine/threonine kinase TBK1 (also known as NAK) (3). Multiple isoforms of ZBP1 are known to exist.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.51ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to 18aa from near the carboxy terminus of human ZBP1 (Genbank accession No. EAW75510).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human ZBP1. Species crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schwartz T, Behlke J, Lowenhaupt K, et al. Structure of the DLM-1-Z-DNA complex reveals a conserved family of Z-DNA- binding proteins. Nat. Struct. Biol. 2001; 8:761-5. 2. Fu Y, Comella N, Tognazzi K, et al. Cloning of DLM-1, a novel gene that is up-regulated in active macrophages, using RNA differential display. Gene 1999; 204:157-63. 3. Takaoda A, Wang Z, Choi MK, et al. DAI (DLM-1/ZBP1) is a cytosolic DNA sensor and an activator of innate immune response. Nature 2007; 448:501-5.