Technical Data
ZO-1 (Tight Junction-associated Polypeptide, Zonula Occludens 1)
ZO-1 is a peripheral membrane protein that is associated with tight junctions (zonula occludentes) which are found on the cytoplasmic surface of epithelial and endothelial cell membranes. ZO-1 has a MW of 225kD and is found in two isoforms that differ by the presence or absence of an 80 amino acid region known as 'motif-a'. These two isoforms are the basis for determining two classes of tight junctions. ZO-1 is known to interact with ZO-2. ZO-1 is homologous to dlg, the Drosophila tumor suppressor. Both are members of a putative signal transduction protein family whose members contain a SH3 domain and a domain homologous to the enzyme guanylate kinase.

Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:.
Optimal dilutions to be determined by the researcher.

Positive Controls for Immunoprecipitation:
Canine MDCK and human Caco-2 cells.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
A 69kD fusion protein corresponding to aa463-1109 of human ZO-1 cDNA. This sequence lies from the N-terminal to the 80aa region (the alpha-motif) present in the a+isoform but absent in the alpha- isoform due to alternative splicing.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Recognizes human ZO-1 alpha+ and ZO-1 alpha- isoforms. Reactivity with dlg has not been characterized. No reactivity with PSD-95 or p55 has been observed on Western Blots, but potential reactivity with these proteins has not been fully characterized. Species Crossreactivtiy: mouse, rat, guinea pig, canine. Moderate reactivity with chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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