Technical Data
Z0750-02B
ZO-1 (Tight Junction-associated Polypeptide, Zonula Occludens 1)
Description:
ZO-1 is a peripheral membrane protein that is associated with tight junctions (zonula occludentes) which are found on the cytoplasmic surface of epithelial and endothelial cell membranes. ZO-1 has a MW of 225kD and is found in two isoforms that differ by the presence or absence of an 80 amino acid region known as 'motif-a'. These two isoforms are the basis for determining two classes of tight junctions. ZO-1 is known to interact with ZO-2. ZO-1 is homologous to dlg, the Drosophila tumor suppressor. Both are members of a putative signal transduction protein family whose members contain a SH3 domain and a domain homologous to the enzyme guanylate kinase.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.5-2ug/ml
Immunoprecipitation: 2-5ug/IP reaction
Immunohistochemistry (Paraffin/Frozen): 2-5ug/ml. To achieve appropriate staining results on formalin-fixed, paraffin-embedded tissue sections, protease pretreatment is required. Use 1-2mg/ml protease and incubate at 37C for 10 minutes. Other enzyme and HIER pre-treatment methods may not be suitable for this antibody. Frozen sections have been successfully stained after fixation with acetone at 4C.
Optimal dilutions to be determined by the researcher.

Positive Controls for Immunoprecipitation:
Canine MDCK and human Caco-2 cells.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.25mg/ml
Immunogen:
A 69kD fusion protein corresponding to aa463-1109 of human ZO-1 cDNA. This sequence lies from the N-terminal to the 80aa region (the alpha-motif) present in the a+isoform but absent in the alpha- isoform due to alternative splicing.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human ZO-1 alpha+ and ZO-1 alpha- isoforms. Reactivity with dlg has not been characterized. No reactivity with PSD-95 or p55 has been observed on Western Blots, but potential reactivity with these proteins has not been fully characterized. Species Crossreactivtiy: mouse, rat, guinea pig, canine. Moderate reactivity with chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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