Technical Data
ZW10 Interactor 1 (ZW10-interacting Protein 1, ZWINT 1, ZWINT1, ZWINT-1, ZW10 Interactor, ZWINT, Human ZW10 Interacting Protein 1, HZwint 1, HZwint-1, KNTC2AP, MGC117174)
This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Immunoprecipitation: 2-5ug/mg lysate
Western Blot: 1:2,000-1:10,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
PabAffinity Purified
100ul4C Do Not FreezeBlue IceHumanRabbit
Region between residue 225 and the C-terminus (residue 277) of human ZW10 Interactor-1 using the numbering given in entry NP_008988.2 (GeneID 11130).
Purified by immunoaffinity chromatography.
Supplied as a liquid in TBS, pH 7.2, 0.1% BSA, 0.09% sodium azide.
Recognizes human ZW10 Interactor-1 protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.