Technical Data
Z1001
Zymolyase 20T Concentrate 50mg/ml, 0.1M Sorbitol (Lyticase, Yeast Lytic Enzyme)
1ml
5x1ml
Molecular Biology Storage: -20°CShipping: Blue Ice
Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is * Lyticase is similar but testing has shown that Zymolyase exhibits greater lytic activity.

See publication by United States Biological titled: A Comparison of Zymolyase, Lyticase and Glusulase.

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below.

Zymolyase 20T Stock Concentrate 50mg/ml is a convenient preparation.

Applications:
Protoplast/Spheroplast Preparation
Yeast Cell Fusion
Yeast Cell Transformation

Appearance: Pale yellow to brown solution/suspension
Activity: 20u/mg

Purity: Purified by ammonium sulfate precipitation.

Unit Definition:
One unit of lytic activity is defined as that amount which indicates 30% of decrease in absorbance at 800nm (A800). Refer to Assay for Enzymatic Activity.

Water: Molecular Biology Grade, RNase, DNase-Free

Filter: 0.2um validated

Sorbitol: 98%

Synonyms: Lyticase*, Yeast Lytic Enzyme

Source: Arthrobacter luteus

Essential Enzyme: b-1,3-glucan laminaripentaohydrolase

Other activities contained:
b-1,3-glucanase: ~1.5x10e3u/mg
Protease: ~15u/mg
Mannanase: ~1.5x10e3u/mg
Amylase, xylanase, phosphatase: Minute amounts
DNase, Rnase: Trace levels reported
(See reference No. 3 as to the definition of each enzyme unit. Each activity varies more of less among lots.)

Optimum pH:
For cell wall lysis: 7.5, 35°C
For yeast glucan hydrolysis: 6.5, 45°C

Stable pH: 5-10

Heat Stability: The lytic activity is lost on incubation at 60°C for 5 minutes.

Properties of Zymolyase:
Specificity (lytic spectrum)(5): Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloekera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomcopsis, Saccharomycodes, Schwanniomyces, etc
Susceptible strains in low concentration (0.2 units/ml):
Ashbya, Endomyces, Kloekera, Kluyveromyces, Pullularia, Saccharomyces
Susceptible strains in high concentration (2.0 units/ml):
Candida, Debaryomyces, Eremothecium, Hansenula, Hanseniaspora, Lipomyces, Metschnikowia, Saccharomycopsis, Saccharomycodes, Schizosaccharomyces, Selenozyma, Trigonopsis, Wickerhamia
Susceptibility depending upon strains:
Bretanomyces, Cryptococcus, Nadsonia, Picia, Rodosporidium, Schwanniomyces, Stephnoascus, Torulopsis
No susceptible strains:
Bullera, Pityosporum, Rhosotorula, Sporidiobolus, Sporobolomyces, Stetigmatomyces, Trichosporon

Activators: SH compound such as cystein, 2-mercaptoethanol of dithiothreitol

Storage and Stability: Stable for 12 months at -20ºC.

Companion Products:
Z1000: Zymolyase 20T
Z1001: Zymolyase 20T Concentrate 50mg/ml, 0.1M Sorbitol
Zymolyase 20T shows 20u/mg of the lytic activity, defined after, toward brewer's yeast cells (Saccharomyces cerevisiae, resting stage) or toward yeast cells of Saccharomyces cerevisiae IFO 0565 cultured statically in malt extract medium (Malt extract 2g, peptone 0.5g, water 100ml) at 20ºC for 34 hours.

Z1004: Zymolyase 100T
Z1005: Zymolyase 100T Concentrate 10mg/ml, 0.1M Sorbitol
Zymolyase 100T, further purified, whose specific activity is 100u/mg.

Note: Zymolyase100T is less soluble than 20T and may not be completely dissolved in buffers. If this is the case use as a suspension.

CAS Number:
37340-57-1
* Lyticase is similar but testing has shown that Zymolyase exhibits greater lytic activity.

See useful publication on the use of USBio’s Zymolyase titled: A Comparison of Zymolyase, Lyticase and Glusulase by Dr. David Burden.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
US Biological Application References: 1. Patel, P.K., et al., Mol. Biol. Cell 17: 308-316 (2006). General References: 1. Kitamura, K., et al., Arch. Biochem. Biophys. 145: 402 (1971). 2. Kitamura, K., et al., J. Gen. Appl. Micro. 153: 403 (1972). 3. Yamamoto, M. & Fukui, S., Agric. Biol. Chem. 41: 1829 (1977). 4. Hsiao, C., J. Carbon, Proc. Natl. Acad. Sci. USA 76: 3829 (1979). 5. Arima, K. & Takano, I., Molec. Gen. Genet. 173: 271 (1979). 6. Sambrook, et al., Molecular Cloning, 2nd Edition, 18.36-18.37 (1989). 7. Ausubel, et al., Current Protocols in Molecular Biology, 2nd Ed., A1-64, 13-12, 13-42.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.