Technical Data
A4000-50D
ATPase PMCA4
Description:
Plasma membrane calcium-ATPase 4 (PMCA4) expression shows little variation across eight drug-treated cell lines and was found to be superior to GAPDH and HPRT1, commonly used reference genes. PMCA4 can be used as a reference gene for normalizing ABC transporter expression in a drug-resistant lung carcinoma cell line. PMCA4 is a good housekeeping gene for normalization of gene expression for polytopic membrane proteins including transporters and receptors.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:15,000
Western Blot: 1:1000
Immunohistochemistry (Frozen & Paraffin): 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG18J323Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified human erythrocyte PMCA ATPase. Epitope: aa51-75. This sequence is common to both PMCA4a and 4b ATPase splice variants.
Purity:
Purified
Form
Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
Specificity:
Recognizes human PMCA4 4 isoforms, 4a and 4b, at ~133 and 129kD, respectively. Species Crossreactivity: porcine and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Filoteo, A.G. et al. Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies. J Biol Chem 272:23741-23747 (1997). Caride, A.J. et al. Detection of isoform 4 of the plasma membrane calcium pump in human tissues by using isoform-specific monoclonal antibodies. Biochem J. 316:353-359 (1996).