Technical Data
ax0013
Axol Neural Progenitor Cells, Human (hNPC), Donor C, Frozen Cells
2M Cells
Biologicals-Cells, Plasma, Serum, Tissues Storage: Liquid N2Shipping: Dry Ice
Axol human neural progenitor cells (hNPCs, ax0011-ax0016) are derived from NIH approved induced pluripotent stem cell (iPSC) lines under a fully defined neural induction condition (Shi, Y. et al, 2012a, b, c). The hNPCs express typical markers of cortical neural stem and progenitor cells, and spontaneously form neural tube-like rosette structures when plated as a monolayer in culture (Fig. 2-6).

Cerebral cortical neurons are implicated in numerous neurological diseases including Alzheimer’s disease, autism, epilepsy and stroke. Despite strong interest in these diseases, high quality human in vitro cell models for cortical neurophysiology were unavailable until now. With Axol hNPCs, we provide neuroscience researchers with a high quality in vitro human system to complement their existing studies of cortical development, function and diseases using animal models.

Advantages of Axol hNPCs:
• Human cells
• Available in industrial quantity
• High purity neural progenitor population
• Ready-made and easy to differentiate
• No reprogramming and differentiation needed
• Robust enough to undergo genetic manipulation
• Supplied with extensive functional and molecular characterization data
• Capable of differentiating to a spectrum of cortical excitatory and inhibitory neurons
• Remain viable in culture for months to facilitate reproducible and long term studies
• Cells from multiple donors are available for purchase allowing data comparison among biological replicates

Applications:
• Studying Corticogenesis in vitro
• Studying neural network formation and signal transmission 
• Disease modelling
• Neurotoxicity testing
• Preclinical drug efficacy testing
• Research and development of cell therapies in animal models

Specifications:
• Quantity: 2 million expandable cells per vial
• Presentation: Cells are provided cryopreserved in 1ml of freezing medium
• Storage Condition: Liquid nitrogen
• Growth Properties: Adherent

Gender:
Male

Donor Age:
Newborn

Starting Material:
Cord Blood CD34+ Cells

Reprogramming Method:
Episomal Plasmid

Neural Induction Method:
Monolayer and Chemically-defined medium

Genetic Modification:
None

Size:
2 Million expandable cells per vial

1. Shi, Y., Kirwan, P., Smith, J., Robinson, H. P. C. & Livesey, F. J. Human cerebral cortex development
from pluripotent stem cells to functional excitatory synapses. Nature neuroscience
15, 477–86, S1 (2012).
2. Shi, Y., Kirwan, P. & Livesey, F. J. Directed differentiation of human pluripotent stem cells to
cerebral cortex neurons and neural networks. Nature Protocols 7, 1836–1846 (2012).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.