		Technical Data
L2499-15	Lipocalin 2 (HNL, NGAL, Neutrophil Gelatinase Associated Lipocalin, Oncogene 24p3, Neutrophil gelatinase-associated lipocalin precursor P25, alpha-2 microglobulin-related subunit of MMP-9)

Description:
Lipocalin 2 is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. Lipocalin 2 is released by activated neutrophils and occurs as 25kD glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B). Low level expression of Lipocalin 2 in a variety of epithelia may be increased in inflammation or cancers. Expression of Lipocalin 2 in the kidney is dramatically increased by acute kidney injury.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: Strong reaction seen when used as a detection antibody in sandwich ELISA in combination with a polyclonal Lipocalin 2 capture antibody and when tested on recombinant Lipocalin 2 coated directly onto the well.
Western Blot: 1:500. Reacts with Lipocalin 2 in both reduced and unreduced forms in Western Blot of SDS-PAGE gels.
Immunohistochemistry: Reacts with Lipocalin 2 when staining paraformaldehyde-fixed sections containing neutrophils.
Optimal dilutions to be determined by the researcher.

Culture Medium:
RPMI 1640 with 10% fetal calf serum.

Hybridoma:
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	6F154	Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Human recombinant Lipocalin 2 in monomer and dimer forms adsorbed to aluminum hydroxide gel.

Purity:

Purified by Protein A/G affinity chromatography

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

Specificity:

Recognizes human Lipocalin 2. Epitope specificity differs from that of clone 6F155. Does not crossreact with Lipocalin 2 from other species.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Kjeldsen L et al; J Biol Chem; 268:10425-32 (1993). 2. Kjeldsen L et al; J Immunol Meth 198:155-64 (1996). 3. Mishra J, et al; Lancet 365(9466):1231-1238 (2005). Biotechnol Bioeng, Aug 20;91(4):502-15 (2005). 4. Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery. Lancet Apr 2-8;365(9466):1231-8 (2005). 5. Ai Zheng; Feb;22(2):143-7 (2003). 6. The neutrophil lipocalin NGAL is a bacteriostatic agent that interferes with siderophore-mediated iron acquisition. Mol Cell Nov;10(5):1033-43 (2002). 7. Clinical manifestations of atherosclerosis in an elderly population are related to plasma neopterin, NGAL and endothelin-1, but not to Chlamydia pneumoniae serology. Int Angiol Jun;21(2):173-9 (2002). 8. The high molecular weight urinary matrix metalloproteinase (MMP) activity is a complex of gelatinase B/MMP-9 and neutrophil gelatinase-associated lipocalin (NGAL). Modulation of MMP-9 activity by NGAL.; J Biol Chem Oct 5;276(40):37258-65 (2001).