Technical Data
L2499-15
Lipocalin 2 (HNL, NGAL, Neutrophil Gelatinase Associated Lipocalin, Oncogene 24p3, Neutrophil gelatinase-associated lipocalin precursor P25, alpha-2 microglobulin-related subunit of MMP-9)
Description:
Lipocalin 2 is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. Lipocalin 2 is released by activated neutrophils and occurs as 25kD glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B). Low level expression of Lipocalin 2 in a variety of epithelia may be increased in inflammation or cancers. Expression of Lipocalin 2 in the kidney is dramatically increased by acute kidney injury.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: Strong reaction seen when used as a detection antibody in sandwich ELISA in combination with a polyclonal Lipocalin 2 capture antibody and when tested on recombinant Lipocalin 2 coated directly onto the well.
Western Blot: Reacts with Lipocalin 2 in both reduced and unreduced forms in Western Blot of SDS-PAGE gels.
Immunohistochemistry: Reacts with Lipocalin 2 when staining paraformaldehyde-fixed sections containing neutrophils.
Optimal dilutions to be determined by the researcher.

Culture Medium:
Hybridoma Serum Free Medium

Hybridoma:
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k6F154Affinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human recombinant Lipocalin 2 in monomer and dimer forms adsorbed to aluminum hydroxide gel.
Purity:
Purified by Protein A/G affinity chromatography
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity:
Recognizes human Lipocalin 2. Epitope specificity differs from that of clone 6F155. Does not crossreact with Lipocalin 2 from other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kjeldsen L, Johnsen AH, Sengeløv H, Borregaard N (1993) J Biol Chem 268:10425-10432. 2. Nielsen BS, Borregaard N, Bundgaard JR, Timshel S, Sehested M, Kjeldsen L (1996) Gut 38:414-420. 3. Matthaeus T, Schulze-Lohoff E, Ichimura T, Weber M, Andreucci M, Park KM, Alessandrini A, Bonventre JV (2001) J Am Soc Nephrol 12:787A. 4. Kjeldsen L, Koch C, Arnljots K, Borregaard N (1996) J Immunol Methods 198:155-164. 5. Tong Z, Kunnumakkara AB, Wang H, Matsuo Y, Diagaradjane P, Harikumar KB, Ramachandran V, Sung B, Chakraborty A, Bresalier RS, Logsdon,C, Aggarwal BB, Krishnan S, Guha S (2008) Canc Res 68: 6100-6108.