Technical Data

029077
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
HRP
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-CCL2 (C-C motif Chemokine 2, Small-inducible Cytokine A2, Monocyte Chemoattractant Protein 1, Monocyte Chemotactic Protein 1, MCP-1, Monocyte Chemotactic And Activating Factor, MCAF, Monocyte Secretory Protein JE, HC11, SCYA2, MCP1) (HRP)

Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Immunogen
Recombinant corresponding to human C-C motif chemokine 2.
Form
Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human CCL2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. "Cloning and sequencing of the cDNA for human monocyte chemotactic and activating factor (MCAF)." Furutani Y., Nomura H., Notake M., Oyamada Y., Fukui T., Yamada M., Larsen C.G., Oppenheim J.J., Matsushima K. Biochem. Biophys. Res. Commun. 159:249-255 (1989). 2. "The human homolog of the JE gene encodes a monocyte secretory protein." Rollins B.J., Stier P., Ernst T., Wong G.G. Mol. Cell. Biol. 9:4687-4695(1989) 3. "Human monocyte chemoattractant protein-1 (MCP-1). Full-length cDNA cloning, expression in mitogen-stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE." Yoshimura T., Yuhki N., Moore S.K., Appella E., Lerman M.I., Leonard E.J. FEBS Lett. 244:487-493 (1989). 4. "Cloning and expression of a gamma-interferon-inducible gene in monocytes: a new member of a cytokine gene family." Chang H.C., Hsu F., Freeman G.J., Griffin J.D., Reinherz E.L. Int. Immunol. 1:388-397 (1989).
USBio References
No references available
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