Technical Data

029191
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-HMGB1 (High Mobility Group Protein B1, High Mobility Group Protein 1, HMG-1, HMG1)

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant corresponding to human HMGB1.
Form
Supplied as a liquid in PBS.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human HMGB1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. "A human placental cDNA clone that encodes nonhistone chromosomal protein HMG-1."Wen L., Huang J.K., Johnson B.H., Reeck G.R.Nucleic Acids Res. 17:1197-1214 (1989). 2. "The active gene that encodes human high mobility group 1 protein (HMG1) contains introns and maps to chromosome 13."Ferrari S., Finelli P., Rocchi M., Bianchi M.E.Genomics 35:367-371 (1996). 3. "Expression of high-mobility group-1 mRNA in human gastrointestinal adenocarcinoma and corresponding non-cancerous mucosa."Xiang Y.-Y., Wang D.-Y., Tanaka M., Suzuki M., Kiyokawa E., Igarashi H., Niato Y., Shen Q., Sugimura H.Int. J. Cancer 74:1-6 (1997).
USBio References
No references available
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