029230
Clone Type
PolyclonalHost
RabbitSource
HumanConjugate
HRPIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-P4HB (Protein Disulfide-isomerase, PDI, Cellular Thyroid Hormone-binding Protein, Prolyl 4-hydroxylase Subunit beta, p55, ERBA2L, PDI, PDIA1, PO4DB) (HRP)
Dsb proteins (DsbA, DsbB, DsbC, and DsbD) catalyze formation and isomerization of protein disulfide bonds in the periplasm of Escherichia coli. Disulphide bond isomerase (DsbC), a member of the thioredoxin superfamily, acts as a disulfide isomerase and converts aberrant disulfide bonds to correct ones. It also acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbC is reoxidized by a yet uncharacterized protein.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Immunogen
Recombinant corresponding to human disulfide-isomerase.
Form
Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human P4HB.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. "Molecular cloning of the beta-subunit of human prolyl 4-hydroxylase. This subunit and protein disulphide isomerase are products of the same gene." Pihlajaniemi T., Helaakoski T., Tasanen K., Myllylae R., Huhtala M.-L., Koivu J., Kivirikko K.I. EMBO J. 6:643-649 (1987). 2. "The nucleotide sequence of a human cellular thyroid hormone binding protein present in endoplasmic reticulum." Cheng S.-Y., Gong Q.-H., Parkison C., Robinson E.A., Appella E., Merlino G.T., Pastan I. J. Biol. Chem. 262:11221-11227 (1987). 3. "Characterization of the human gene for a polypeptide that acts both as the beta subunit of prolyl 4-hydroxylase and as protein disulfide isomerase." Tasanen K., Parkkonen T., Chow L.T., Kivirikko K.I., Pihlajaniemi T. J. Biol. Chem. 263:16218-16224 (1988).USBio References
No references available