Technical Data

029363
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
HRP
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-SNRPA (U1 Small Nuclear Ribonucleoprotein A, U1 snRNP A, U1-A, U1A) (HRP)

Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Immunogen
Recombinant corresponding to human U1 small nuclear ribonucleoprotein A.
Form
Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human SNRPA.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. "Structure, chromosomal localization and evolutionary conservation of the gene encoding human U1 snRNP-specific A protein." Nelissen R.L.H., Sillekens P.T.G., Beijer R.P., Geurts van Kessel A.H.M., van Venrooij W.J. Gene 102:189-196 (1991). 2. "cDNA cloning of the human U1 snRNA-associated A protein: extensive homology between U1 and U2 snRNP-specific proteins." Sillekens P.T.G., Habets W.J., Beijer R.P., van Venrooij W.J. EMBO J. 6:3841-3848 (1987). 3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127 (2004).
USBio References
No references available
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