Rabbit Anti-ATG4D, NT (Cysteine Protease ATG4D, AUT-like 4 Cysteine Endopeptidase, Autophagin-4, Autophagy-related Cysteine Endopeptidase 4, Autophagy-related Protein 4 Homolog D, APG4D, AUTL4) (PE)
Atg4D is a member of the Atg4 family of C54 endopeptidases that are required for the synthesis of autophagosomes. Atg4D is found to be cleaved by caspase-3 into a more active form where it can interact with the autophagosome marker Atg8. Atg4D is also known to be associated with mitochondrial dysfunction and the induction of apoptosis.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Immunohistochemistry: 1:200 Optimal dilutions to be determined by the researcher.
Positive Control
A431 cell lysate
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic linear peptide corresponding to the N-terminus of human ATG4D (KLH). Species sequence homology: rhesus macaque (100%).
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by affinity chromatography.
Specificity
Recognizes human ATG4D. Species Crossreactivity: Mouse and rat.
