Technical Data

029442
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2a,k
Clone Number
BV6
Grade
Affinity Purified
Applications
FC IC IHC WB
Crossreactivity
Hu
Accession #
NP_001786
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CDH5 (Cadherin-5, 7B4 Antigen, Vascular Endothelial Cadherin, VE-cadherin, CD144)

Human Vascular Endothelial (VE)-cadherin is a calcium-dependent adhesion molecule strictly located at cell-to-cell junctions. VE-cadherin is present in all types of endothelium (veins, arteries, capillary and large vessels). Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells. Cadherins may thus contribute to the sorting of heterogeneous cell types. VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. VE-cadherin also associates with alpha-catenin forming a link to the cytoskeleton.

Applications
Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:2000 Immunohistochemistry (Formalin fixed paraffin embedded): 1:10-1:20 Immunocytochemistry: 1:500 Optimal dilutions to be determined by the researcher.
Positive Control
HUVEC cell lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
HUVEC cells
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human VE-Cadherin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Prigozhina, N.L., et al. (2007). Assay Drug Dev Technol. 5(1):29-48. 2. Cohen, C.D., et al. (2006). Proc Natl Acad Sci USA. 103(15):5682-5687. 3. Wary, K.K., et al. (2003). Mol Cancer. 2(1):25. 4. Humtsoe, J.O., et al. (2003). EMBO J. 22(7): 1539-1554. 5. Herren, B., et al. (1998). Mol Biol Cell. 9(6): 1589-1601. 6. Harraz, M., et al. (2001). Stem Cells. 19:304- 312. 7. Yang, S., et al. (1999). American Journal of Pathology. 155:887-895. 8. Reyes, M., et al. (2002). J Clin Invest. 109:337-346. 9. Matsumoto, Y., et al. (1993). Transplantation. 56(1):69-75. 10. Horak, E.R., et al. (1992). Lancet. 340(8828): 1120-1124. 11. Gerecht-Nir, S., et al. (2003). Lab Invest. 83(12):1811-1820. 12. Konstantoulaki, M., et al. (2003). AmJ Physiol Lung Cell Mol Physiol. 285:L434-L442. 13. Martin-Padura, I., et al. (1995) J. Pathol. 175(1):51-57. 14. Lampugnani, M.G., et al. (1992). J. Cell Biol. 118:1511-1522. 15. Tang, D.G., et al. (1993). J. Biol. Chem. 268(30):22883-22894. 16. Brevario, F., et al. (1995). Aterioschler Thromb Vasc Biol. 15(8):1229-1239.
USBio References
No references available
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