Rabbit Anti-Cholesterol Acyltransferase 1 (Sterol O-acyltransferase 1, Acyl-coenzyme A:cholesterol Acyltransferase 1, ACAT-1, SOAT1, ACACT, ACACT1, ACAT, ACAT1, SOAT, STAT) (MaxLight 550)
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
Cholesterol acyltransferase 1 is mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA and plays a significant role in ketone body metabolism. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. Recent studies suggests that ACAT1 may be a therapeutic target for treating certain forms of Alzeheimer's disease.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.05ug/ml Immunohistochemistry: 1:1000 Optimal dilutions to be determined by the researcher.
Positive Control
Caco-2 cell lysate
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic linear peptide corresponding to human Cholesterol acyltransferase 1 (KLH).
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Cholesterol Acyltransferase 1. Species sequence homology: Rhesus macaque, chimpanzee, rat, mouse (93%).
