Technical Data

029497
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Serum
Applications
ChIP WB
Crossreactivity
Hu
Accession #
NM_002107.3
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Histone H3, CT (Histone H3.1, H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ) (Not for Export EU)

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Applications
Suitable for use in Western Blot and Chromatin Immunoprecipitation. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Positive Control
Includes normal rabbit serum and primers specific for human B-globin
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to the C-terminus of human Histone H3 (KLH).
Form
Supplied as a liquid in 0.02M phosphate, 0.25M sodium chloride, 0.09% sodium azide, 30% glycerol.
Purity
Serum
Specificity
Recognizes human Histone H3. Species sequence homology: Mouse, rat, yeast, chicken.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Chik, C L, et al. (2007). Endocrinology. 148: 1465-1472. 2. Eckey, Maren, et al. (2007). Mol Cell Biol. (10):3557-3568. 3. Morris, Stephanie A, et al. (2007). J Biol Chem. 282: 7632-7640. 4. Wang, Xin, et al. (2007). EMBO J. 26: 1934-1941. 5. Kizer, Kelby O, et al. (2005). Mol Cell Biol. 25: 3305-3316.
USBio References
No references available
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