Rabbit Anti-INF2 (Inverted Formin-2, HBEBP2-binding Protein C, C14orf151, C14orf173) (APC)
NF2 (inverted formin 2) is a mouse formin that has been found to interact with actin through the C-terminus to the formin homology 2 domain. The WASP homology 2 motif activates another region that appropriates actin monomers. INF2 has a unique ability to speed up polymerization and depolymerization of actin filaments. Mutations in the N-terminal diaphanous inhibitory domain of INF2 have been proven to be a main cause of familial focal segmental glomerulosclerosis.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate buffer, pH 6.0 is recommended. Optimal dilutions to be determined by the researcher.
Positive Control
HeLa cell lysate
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic linear peptide corresponding to human INF2, conjugated to KLH. Species Sequence Homology: mouse, 68%.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by affinity chromatography.
Specificity
Recognizes human INF2. Species Crossreactivity: rat.
