Rabbit Anti-INF2 (Inverted Formin-2, HBEBP2-binding Protein C, C14orf151, C14orf173)
NF2 (inverted formin 2) is a mouse formin that has been found to interact with actin through the C-terminus to the formin homology 2 domain. The WASP homology 2 motif activates another region that appropriates actin monomers. INF2 has a unique ability to speed up polymerization and depolymerization of actin filaments. Mutations in the N-terminal diaphanous inhibitory domain of INF2 have been proven to be a main cause of familial focal segmental glomerulosclerosis.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 0.05ug/ml, detected INF2 in 10ug of HeLa cell lysate. Immunohistochemistry (Paraffin): 1:500, detected INF2 in stomach cancer tissue. Requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate buffer, pH 6.0 is recommended. Optimal dilutions to be determined by the researcher.
Positive Control
HeLa cell lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic linear peptide corresponding to human INF2, conjugated to KLH. Species Sequence Homology: mouse, 68%.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human INF2. Species Crossreactivity: rat.
