Rabbit Anti-KEAP1 (Kelch-like ECH-associated Protein 1, Cytosolic Inhibitor of Nrf2, INrf2, Kelch-like Protein 19, INRF2, KIAA0132, KLHL19) (HRP)
Anti-oxidant defense is known to be regulated by various mechanisms including the usage of nuclear factor erythroid 2-related factor 2 (Nrf2). Under normal physiological conditions, Nrf2 is bound to Kelch-like ECH-associated protein 1 (Keap1), a cytoplasmic repressor. Keap1 also acts as a substrate adaptor for Cullin3-dependent ubiquitin ligase and also targets Nrf2 for degradation by proteosomes. The substrate adaptor function of Keap1 is inactivated during physiological stress. In this respective condition, a range of oxidative and electrophilic stimuli such as ROS, diethyl malonate, and certain disease processes, resulting from Nrf2 accumulation, enters the nucleus and thereby activates the expression of select anti-oxidant genes. Such genes promote the detoxification of ROS and other harmful molecules, which contribute to the carcinogenesis.
Applications
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Positive Control
HeLa cell lysate
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic linear peptide corresponding to human KEAP1, conjugated to KLH.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish peroxidase (HRP).
Purity
Purified by affinity chromatography.
Specificity
Recognizes human KEAP1. Species Crossreactivity: mouse and rat.
