Technical Data

029553
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IC WB
Crossreactivity
Hu Mo Rt
Accession #
NP_001010866.1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-NET5 (Transmembrane Protein 201, Spindle-associated Membrane Protein 1, TMEM201, SAMP1)

Transmembrane protein 201, also known as NET5, is a transmembrane protein of the nuclear envelope that is homologous to spindle-associated membrane protein 1 (SAMP1) in humans. NET5 is thought to be expressed on the inner nuclear membrane during interphase and move to the polar regions of the mitotic spindle during mitosis.

Applications
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1ug/ml detected NET5 on 10ug of rat heart tissue lysate. Optimal dilutions to be determined by the researcher.
Positive Control
Rat heart tissue lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated linear peptide corresponding to human NET5. Species Sequence Homology: Orangutan
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide
Purity
Purified by affinity chromatography
Specificity
Recognizes human NET5. Species Crossreactivity: mouse and rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Buch, C., et al. (2009). J Cell Sci. 122:2100- 2107. 2. Wilkie, G., et al. (2011). Mol. Cell Proteomics. 10(1) M110.003129-1-16.
USBio References
No references available
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