Rabbit Anti-NRXN1 (Neurexin-1-alpha, Neurexin I-alpha, KIAA0578) (MaxLight 490)
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
Neurexins are heavily glycosylated and processed neuronal transmembrane cell surface proteins likely involved in cell recognition and adhesion. Antibody Anti-Neurexin-1-alpha was made against a peptide immunogen corresponding to a stretch of aa’s in the extracellular domain of human Neurexin-1-alpha. Although the theoretical full length of Neurexin-1-alpha is 161kD, the protein detected in human brain lysates may show a band around 160kD and additional bands around 45kD as a possible result of the posttranslational processing previously reported.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.1ug/ml Immunohistochemistry: 1:100 Optimal dilutions to be determined by the researcher.
Positive Control
Human cerebellum tissue lysate
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic linear peptide corresponding to human Neurexin-1-alpha at the extracellular laminin G-like 3 domain, conjugated to KLH. Species Sequence Homology: chimpanzee, Rhesus Macaque, canine, bovine, 100%
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Neurexin-1-alpha. |Species Crossreactivity: mouse and rat
