Rabbit Anti-PP6R3 (Serine/Threonine-protein Phosphatase 6 Regulatory Subunit 3, SAPS Domain Family Member 3, Sporulation-induced Transcript 4-associated Protein SAPL, PPP6R3, C11orf23, KIAA1558, SAPL, SAPS3) (MaxLight 550)
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
PP6R3 also known as SAPS3 is a regulatory subunit of protein phosphatase 6 which is important for cell survival and is associated with TOR signaling in yeast. PP6R3 has been implicated in the transition from G1 to the S phase of mitosis. The PP6R3 subunit is one of three human versions of SAPs which may also have a role to play in regulation of NF-kappaB.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: detected PP6R3 on 10ug of HeLa cell lysate. Immunohistochemistry: using PP6R3 in human kidney and human breast tissues. Optimal dilutions to be determined by the researcher.
Positive Control
HeLa cell lysate
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant corresponding to human PP6R3 fused to His-tag.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human PP6R3.
