029611-PE
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
PEIsotype
IgG1,kClone Number
20C3.2Grade
Affinity PurifiedApplications
IC WBCrossreactivity
Hu RtAccession #
NP_001122316Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-SMARCA4 (Transcription Activator BRG1, ATP-dependent Helicase SMARCA4, BRG1-associated Factor 190A, BAF190A, Mitotic Growth and Transcription Activator, Protein BRG-1, Protein Brahma Homolog 1, SNF2-beta, SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily A Member 4, BAF190A, BRG1, SNF2B, SNF2L4) (PE)
Also known as Protein BRG1 and SNF2-beta, among other aliases, SMARCA4 a transcriptional activator belonging to the SNF2/RAD54 helicase family, and acts as a component of the SWI/SNF ATP-dependent chromatin remodeling complexes family. BRG1 contains 4 domains of interest; a bromo domain, a helicase ATP-binding domain, a helicase C-terminal domain, and a HAS domain. It is a component of several different complexes with distinctive roles such as the CREST-BRG1 complex which regulates the activation of promoters in a calcium-dependent manner, releasing repressor complexes and recruiting activator complexes. It is also a part of the nBAF and npBAF complexes which are involved in the chromatin remodeling of mononucleosomes and nucleosomal arrays. As a component of the SWI/SNF complex, SMARCA4 has a role in tumor suppression and has been shown to regulate cell cycle and differentiation when present in neoplasma.
Applications
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.5ug/ml Immunocytochemistry: 1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
HeLa nuclear extract
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human SMARCA4 fused to GST-tag.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human SMARCA4. Species Crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Schneppenheim, R., et al. (2010). Am J Hum Genet. 86(2):279-284. 2. Fujimoto, M., et al. (2010). Mol Biol Cell. 21(1):106-116. 3. Zager, R.A., and Johnson, A.C. (2009). Am J Physiol Renal Physiol. 296(5):F1032-1041. 4. Kenneth, N.S., et al. (2009). J Biol Chem. 294(7):4123-4131. 5. Voss, T.C., et al. (2009). J Cell Sci. 122(Pt 3):345-356. 6. Osipovich, O., et al. (2007). Nat Immunol. 8(8):809-816. 7. Dammer, E.B., Leon, A., and Sewer, M.B. (2007). Mol Endocrinol. 21(2):415-438. 8. Ramirez-Carrozzi, V.R., et al. (2006). Genes Dev. 20(3):282-296. 9. Sif, S, et al. (1998). Genes Dev. 12(18):2842- 2851. 10. Schnitzler, G., Sif, S., and Kingston, R.E. (1998). Cell. 94(1):17-27.USBio References
No references available