Rabbit Anti-TRIC-A (Trimeric Intracellular Cation Channel Type A, TRICA, Transmembrane Protein 38A, TMEM38A) (MaxLight 550)
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
TRIC-A ), also known as Tmem38A, and sometimes NET1, is one of a number of TRIC channel subtypes that are believed to function as a Ca2+ counter-ion channel. TRIC-A is highly expressed in excitable cells and seems to work in coordination with Ca2+ release to balance transient negative potential. TRIC-A is located on the sarcoplasmic reticulum (SR) and is thought to be regulated by trans-membrane voltage. TRIC-A deficiency may lead to an abundance of Ca2+ and cause volatility in Ca2+ movement across the SR membrane.
Applications
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human fetal skeletal muscle tissue lysate
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic linear peptide corresponding to the cytoplasmic domain of human TRIC-A (KLH).
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human TRIC-A. Species Crossreactivity: Rat and mouse. Species sequence homology: Chimpanzee (100%).
