Technical Data

029925
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
11G12C11
Grade
Ascites
Applications
E IHC WB
Crossreactivity
Hu
Gene ID
1978
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-4E-BP1
BP-1; 4EBP1; 4E-BP1; PHAS-I; MGC4316; EIF4EBP1

4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13kD). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24kD), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (paraffin): 1:200-1:1000. Western Blot: 1:500-1:2000. ELISA: 1:10,000. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant fragment of human 4EBP1 expressed in E. coli.
Form
Supplied as a liquid, 0.03% sodium azide.
Purity
Ascites
Specificity
Recognizes human 4EBP1

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Pause, A. et al. 1994.Nature. 371:762–767.|2. Fadden, P. et al. 1997. J. Biol. Chem. 272:10240–10247.|
USBio References
No references available
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