Technical Data

030024
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
13B102
Grade
Purified
Applications
E FC IHC WB
Crossreactivity
Hu Mk Mo
Gene ID
6347
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CCL2 (C-C motif chemokine 2)
HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

Applications
Western Bloting: 1:500-1:2000. Immunohistochemistry: 1:200-1:1000. Immunofluorescence: 1:200-1:1000. Flow cytometry: 1:200-1:400. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant fragment of human CCL2 expressed in E. coli.
Form
Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer.
Purity
Purified
Specificity
Human, Mouse, Monkey

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
J Cereb Blood Flow Metab. 2010 Mar;30(3):459-73. |Prostate. 2010 Mar 1;70(4):433-42.|
USBio References
No references available
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